THE MONOTRACE ADVANTAGE（優(yōu)勢(shì)）

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of allergen protein contamination in a variety of food samples. MonoTrace ELISAs incorporate the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

1、Target a major allergen resistant to food processing effects

2、Recognize a specific target, thereby eliminating cross-reactivity and reducing the possibility?

? of‘false positive’results

3、Perform well in a wide variety of food products with negligible food matrix interference

BioFront Technologies開(kāi)發(fā)了第一條完全以單克隆抗體為基礎(chǔ)的檢測(cè)試劑盒產(chǎn)品（MonoTrace ELISAs），檢測(cè)各種食物基質(zhì)中的致敏蛋白污染。MonoTrace ELISAs綜合使用一系列精準(zhǔn)確定的經(jīng)過(guò)嚴(yán)格篩選過(guò)程后仔細(xì)挑選的單克隆抗體來(lái)實(shí)現(xiàn)：

1、分析耐食物加工的主要過(guò)敏原

2、特異性識(shí)別目標(biāo)成分，從而消除交叉反應(yīng)，以及減少“假陽(yáng)性”結(jié)果的可能性

3、在各種食品（食品基質(zhì)干擾可以忽略不計(jì)）的檢測(cè)中表現(xiàn)優(yōu)異

BUCKWHEAT?ASSAY PERFORMANCE

蕎麥分析試劑盒性能

Overall Assay Time: Now just 30 minutes post-extraction!

Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.

Number of Tests per Kit: 96

Limit of Detection: ＜1 ppm

Range of Quantification: 1 to 40 ppm

Specificity: Buckwheat

*= calculations of parameters are based on representative data from ten assays, however, users should consult accompanying certificate of analysis for lot-specific values.

實(shí)驗(yàn)總時(shí)間：現(xiàn)在抽提后僅需30分鐘！

孵育時(shí)間：抽提，樣品孵育，二抗孵育，底物孵育每步僅需10分鐘。

每一試劑盒測(cè)試數(shù)量：96

*檢測(cè)限：＜1 ppm

*定量范圍：1至40 ppm

特異性：蕎麥

*=參數(shù)的計(jì)算根據(jù)10次代表性的數(shù)據(jù)得出，然而，使用者應(yīng)當(dāng)查閱附隨的分析證書中的具體批次數(shù)值。

CROSS-REACTIVITY 交叉反應(yīng)

At concentrations as high as 100,000 ppm,? no cross-reactivity?was observed in a large panel of assayed samples, including:?sesame seed, mustard seed, sunflower seed, soy, lentils, green pea, chickpea, kidney beans, lima beans, walnut, hazelnut, peanut, pistachio, cashew, macadamia nut, pine nut, coconut, chestnut, Brazil nut, and pine nut.

在濃度高達(dá)100,000 ppm時(shí)，對(duì)一份大型分析樣品中沒(méi)有觀察到交叉反應(yīng)，包括：芝麻籽，芥菜籽，葵花籽，大豆，扁豆，綠豌豆，鷹嘴豆，菜豆，利馬豆，核桃，榛子，花生，開(kāi)心果，腰果，夏威夷果，松子，椰子，板栗，巴西堅(jiān)果，松子。

RECOVERY 回收

Recovery of spiked buckwheat samples at various concentrations was efficient (>90%) when the following food matrices were assayed: chocolate, cereals, ice cream, cake mix

當(dāng)分析以下食物基質(zhì)時(shí)，不同濃度蕎麥樣品的加標(biāo)回收率很高（>90%）：巧克力，麥片，面冰激凌，蛋糕。