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Chroma 激光專用濾光片 FISH濾光片 TIRF濾光片 Raman濾光片

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  • 品　　牌：
  • Chroma
  • 主要規(guī)格：
  • FISH濾光片 TIRF濾光片 Raman濾光片
  • 用　　途：
  • 用于顯微鏡，觀察FISH TIRF Raman現(xiàn)象
    • Chroma激光專用濾光片 FISH濾光片 TIRF濾光片 Raman濾光片是常見的集中濾光片類型，屬于對光學品質(zhì)要求較高的濾光片。 激光專用濾光片用于激光作為光源的顯微鏡，例如激光掃描共聚焦熒光顯微鏡（LSCM），全內(nèi)反射濾光片（TIRF）用于全內(nèi)反射熒光顯微鏡（TIRFM），拉曼濾光片用于拉曼成像（Raman），全內(nèi)反射熒光片用于熒光原位雜交分析（FISH）。 一、熒光顯微鏡 LSCM顯微鏡 TIRFM顯微鏡 FISH熒光原為雜交法 Raman光譜分析 熒光顯微鏡(Fluorescence microscope) ： 熒光顯微鏡是以紫外線為光源， 用以照射被檢物體， 使之發(fā)出熒光， 然后在顯微鏡下觀察物體的形狀及其所在位置。熒光顯微鏡用于研究細胞內(nèi)物質(zhì)的吸收、運輸、化學物質(zhì)的分布及定位等。 細胞中有些物質(zhì)，如葉綠素等，受紫外線照射后可發(fā)熒光；另有一些物質(zhì)本身雖不能發(fā)熒光，但如果用熒光染料或熒光抗體染色后，經(jīng)紫外線照射亦可發(fā)熒光，熒光顯微鏡就是對這類物質(zhì)進行定性和定量研究的工具之一。 激光掃描共聚焦熒光顯微鏡(laser scanning confocal microscopy,LSCM) 測針孔和光源針孔始終聚焦于同一點，使聚焦平面以外被激發(fā)的熒光不能進入檢測針孔。 激光共聚焦的工作原理簡單表達就是它采用激光為光源，在傳統(tǒng)熒光顯微鏡成像的基礎上，附加了激光掃描裝置和共軛聚焦裝置，通過計算機控制來進行數(shù)字化圖像采集和處理的系統(tǒng)。 TIRFM 全內(nèi)反射熒光顯微鏡 全內(nèi)角反射熒光顯微鏡（total internal reflection fluorescence microscope，TIRFM），利用光線全反射后在介質(zhì)另一面產(chǎn)生衰逝波的特性，激發(fā)熒光分子以觀察熒光標定樣品的極薄區(qū)域，觀測的動態(tài)范圍通常在200 nm以下。因為激發(fā)光呈指數(shù)衰減的特性，只有極靠近全反射面的樣本區(qū)域會產(chǎn)生熒光反射，大大降低了背景光噪聲干擾觀測標的，能幫助研究者獲得高質(zhì)量的成像質(zhì)量和可靠的觀測數(shù)據(jù)。故此項技術廣泛應用于細胞表面物質(zhì)的動態(tài)觀察。 二、激光專用濾光片 FISH濾光片 FITR濾光片 Raman濾光片 NF濾光片的定義 ND濾光片 中性灰度鏡（neutral density filter）又叫中灰密度鏡，簡稱ND鏡，其作用是過濾光線。這種濾光作用是非選擇性的，也就是說，ND鏡對各種不同波長的光線的減少能力是同等的、均勻的，只起到減 弱光線的作用，而對原物體的顏色不會產(chǎn)生任何影響，因此可以真實再現(xiàn)景物的反差。 FISH濾光片 熒光原位雜交技術（Fluorescence in situ hybridization, FISH）是根據(jù)已知微生物不同分類級別上種群特異的DNA序列，以利用熒光標記的特異寡聚核苷酸片段作為探針，與環(huán)境基因組中DNA分子雜交，檢測該特異微生物種群的存在與豐度。 NF濾光片 陷波濾光片，通常也被稱作帶阻或者帶抑制濾光片。它可以透過絕大多數(shù)波長，但是將特定波長范圍內(nèi)（阻帶）的光衰減到非常低的水平。拉曼光譜用。 Chroma激光專用濾光片 FISH濾光片 FITR濾光片 Raman濾光片的型號參數(shù) 熒光濾光片型號 濾光片類型 濾光片說明 注釋（燃料，長通或短通） Blue-27M AS 27mm diameter, mounted in 1.25" threaded ring Ha/8-27M AS 27mm diameter, mounted in 1.25" threaded ring Lum-27M AS 27mm diameter, mounted in 1.25" threaded ring Red-27M AS 27mm diameter, mounted in 1.25" threaded ring Green-27M AS 27mm diameter, mounted in 1.25" threaded ring Ha/8-31 AS 31mm diameter, unmounted Red-31 AS 31mm diameter, unmounted Blue-31 AS 31mm diameter, unmounted Green-31 AS 31mm diameter, unmounted Lum-31 AS 31mm diameter, unmounted Lum-36 AS 36mm diameter, unmounted Green-36 AS 36mm diameter, unmounted Blue-36 AS 36mm diameter, unmounted Red-36 AS 36mm diameter, unmounted Ha/8-36 AS 36mm diameter, unmounted Red-50 AS 50mm diameter, unmounted Lum-50 AS 50mm diameter, unmounted Ha/8-50 AS 50mm diameter, unmounted Green-50 AS 50mm diameter, unmounted Green-50S AS 50mm x 50mm, square Blue-50S AS 50mm x 50mm, square Ha/8-50S AS 50mm x 50mm, square Lum-50S AS 50mm x 50mm, square Red-50S AS 50mm x 50mm, square Ha/8-65S AS 65mm x 65mm, square Red-65S AS 65mm x 65mm, square Lum-65S AS 65mm x 65mm, square Blue-65S AS 65mm x 65mm, square Green-65S AS 65mm x 65mm, square Green-27M AS 27mm diameter, mounted in 1.25" threaded ring Red-27M AS 27mm diameter, mounted in 1.25" threaded ring Lum-27M AS 27mm diameter, mounted in 1.25" threaded ring Blue-27M AS 27mm diameter, mounted in 1.25" threaded ring Lum-31 AS 31mm diameter, unmounted Green-31 AS 31mm diameter, unmounted Blue-31 AS 31mm diameter, unmounted Red-31 AS 31mm diameter, unmounted Red-36 AS 36mm diameter, unmounted Blue-36 AS 36mm diameter, unmounted Lum-36 AS 36mm diameter, unmounted Green-36 AS 36mm diameter, unmounted Green-50 AS 50mm diameter, unmounted Lum-50 AS 50mm diameter, unmounted Red-50 AS 50mm diameter, unmounted Red-50S AS 50mm x 50mm, square Lum-50S AS 50mm x 50mm, square Green-50S AS 50mm x 50mm, square Blue-50S AS 50mm x 50mm, square Green-65S AS 65mm x 65mm, square Blue-65S AS 65mm x 65mm, square Red-65S AS 65mm x 65mm, square Lum-65S AS 65mm x 65mm, square HQ493/16x EX Spectrum Green? HQ527/30m EM Spectrum Green? Q507lp BS Spectrum Green? HQ548/20x EX Spectrum Orange? HQ580/14m EM Spectrum Orange? Q565lp-AR BS Spectrum Orange? 400dclp-AR BS DAPI D470/40m-AR EM DAPI AT350/50x EX DAPI HQ522/40m EM FITC Q497lp BS FITC HQ471/39x EX FITC Q557lp BS Cy3? v1 HQ567/15m EM Cy3? v1 HQ546/11x EX Cy3? v1 HQ565/18m EM Cy3? v2 HQ530/30x EX Cy3? v2 Q553lp BS Cy3? v2 Q593lp BS Cy3.5? v1 HQ581/10x EX Cy3.5? v1 HQ617/40m EM Cy3.5? v1 HQ617/40m EM Cy3.5? v2 HQ580/13x EX Cy3.5? v2 Q593lp BS Cy3.5? v2 Q647lp BS Cy5? v1 HQ622/36x EX Cy5? v1 HQ667/30m EM Cy5? v1 HQ667/30m EM Cy5? v2 HQ630/20x EX Cy5? v2 Q649lp BS Cy5? v2 Q697lp BS Cy5.5? HQ682/12x EX Cy5.5? HQ721/42m EM Cy5.5? HQ740/35x EX Cy7? HQ795/50m EM Cy7? Q765lp BS Cy7? Q615lp BS Texas Red? HQ631/23m EM Texas Red? HQ602/13x EX Texas Red? HQ782/20x EX Laser-Pro HQ817/25m EM Laser-Pro Q800lp BS Laser-Pro ET460/50m EM ET - 405nm Laser Bandpass Set for TIRF applications ZET405/20x EX ET - 405nm Laser Bandpass Set for TIRF applications ZT405rdc BS ET - 405nm Laser Bandpass Set for TIRF applications ET425lp EM ET - 405nm Laser Bandpass Set for TIRF applications ET460lp EM ET - 440nm Laser Bandpass Set for TIRF applications ZET436/20x EX ET - 440nm Laser Bandpass Set for TIRF applications ZT442rdc BS ET - 440nm Laser Bandpass Set for TIRF applications ET480/40m EM ET - 440nm Laser Bandpass Set for TIRF applications ZT442rdc BS ET - 440nm Laser Bandpass Set for TIRF applications ET500/40m EM ET - 440nm Laser Bandpass Set for TIRF applications ZET436/20x EX ET - 440nm Laser Bandpass Set for TIRF applications ET460lp EM ET - 440nm Laser Bandpass Set for TIRF applications ZT488rdc BS ET - 488nm Laser Bandpass Set for TIRF applications ET500lp EM ET - 488nm Laser Bandpass Set for TIRF applications ET525/50m EM ET - 488nm Laser 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applications ET665lp EM ET - 640-647nm Laser Longpass Set for TIRF applications ET705/72m EM ET - 640-647nm Laser Longpass Set for TIRF applications ZT405/488rpc BS ET – 405/488nm Laser Dual Band Set for TIRF applications ZET405/488x EX ET – 405/488nm Laser Dual Band Set for TIRF applications ZET405/488m-TRF EM ET – 405/488nm Laser Dual Band Set for TIRF applications ZET405/488x EX ET – 405/488nm Laser Dual Band Set for TIRF applications ZET405/488m EM ET – 405/488nm Laser Dual Band Set for TIRF applications ZT405/488rpc BS ET – 405/488nm Laser Dual Band Set for TIRF applications ET450/50m EM ET – 405/488nm Laser Dual Band Set for TIRF applications ET525/50m EM ET – 405/488nm Laser Dual Band Set for TIRF applications ZET442/514m-TRF EM ET – 442/514nm Laser Dual Band Set for TIRF applications ZT442/514rpc BS ET – 442/514nm Laser Dual Band Set for TIRF applications ZET442/514x EX ET – 442/514nm Laser Dual Band Set for TIRF applications ZET442/514x EX ET – 442/514nm Laser Dual Band Set 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ZET532/640x EX ET – 532/640nm Laser Dual Band Set for TIRF applications ZET532/640m EM ET – 532/640nm Laser Dual Band Set for TIRF applications ET655lp EM ET – 532/640nm Laser Dual Band Set for TIRF applications ZET405/488/561x EX ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation ZT405/488/561rpc BS ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation ET575lp EM ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation ZET488/561m EM ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation ET525/50m EM ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation ZT405/488/561rpc BS ET - 405/488/561nm Laser Triple Band Set for TIRF applications ZET405/488/561x EX ET - 405/488/561nm Laser Triple Band Set for TIRF applications ZET405/488/561m-TRF EM ET - 405/488/561nm Laser Triple Band Set for TIRF applications ZET405/488/561m EM ET - 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Set for TIRF applications ET700/50m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications ZET405/488/561/647m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications ZET405/488/561/647x EX ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications ZT405/488/561/647rpc BS ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications ET450/50m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications ET525/50m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications ET605/52m EM ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications

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