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phyb/抗植物色素b抗體

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  • 品　　牌：
  • PHYTOAB
  • 主要規(guī)格：
  • 150ug
  • 用　　途：
  • WB
    • PHYB / Anti-Phytochrome B antibody Product Information Description: Mouse monoclonal (Clone: 10P5) antibody Background: PHYB is a red/far-red photoreceptor involved in the regulation of de-etiolation. It exists in two inter-convertible forms: Pr and Pfr (active). It is involved in the light-promotion of seed germination and in the shade avoidance response. Synonyms: PHYB, HY3, OOP1, OUT OF PHASE 1, PHYTOCHROME B Immunogen: Recombinant protein of PHYB derived from Arabidopsis thaliana AT2G18790. Form: Lyophilized Quantity: 150 μg Purification: Protein A purified Reconstitution: Reconstitution with 150μl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube". Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution. Shipping: The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. Application Information Applications: Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user. Expected Results: 125 kDa Confirmed Reactivity: Arabidopsis thaliana Predicted Reactivity: For more species homologues information, please contact tech support at tech@phytoab.com. Application Example Lane 1: 35 μg total protein from phyB-9 mutant of Arabidopsis thaliana leaf. Lane 2: 35 μg total protein from Arabidopsis thaliana leaf. Electrophoresis: 8% SDS-Urea-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1h. Blocking: 5% skim milk at RT or 4℃ for 1h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP). Detection: using chemiluminescence substrate and image were captured with CCD camera.
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